Polymerase Chain Reaction

For a 50 µl reaction, use:

5 µl 10x PCR buffer

7 µl 25 mM MgCl2

2 µl dNTP

1 µl primer A

1 µl primer B

1 µl Taq polymerase

1 µl template DNA

32 µl ddH2O

10x PCR buffer:

5 parts 1 M KCl

1 part 1 M Tris, pH 8.0

4 parts ddH2O